Vanda tricolor is one of the most valuable ornamental orchid species. However, there is a time constrain and some difficulties if Vanda is cultivated in conventional way. In vitro culture technique is an alternative solution to produce large quantity of Vanda shoot in relatively short period; i.e. by using system of TIS (temporary immersion system) RITA© and flask thin layer medium culture. This research was conducted to apply the TIS method to propagate Vanda shoot for the first time, as well as to evalua