Suspended nanoparticles in conventional fluids, called nanofluids, have been the subject of intensive study worldwide since pioneering researchers recently discovered the anomalous thermal behavior of these fluids. The heat transfer from smaller area is achieved through microchannels. The heat transfer principle states that maximum heat transfer is achieved in microchannels with minimum pressure drop across it. In this research work the experimental and numerical investigation for the improved heat transfer characteristics of serpentine shaped microchannel heat sink using Al2O3/water nanofluid is done. The fluid flow characteristics is also analyzed for the serpentine shaped micrchannel. The experimental results of the heat transfer using Al2O3 nanofluid is compared with the numerical values. The calculations in this work suggest that the best heat transfer enhancement can be obtained by using a system with an Al2O3–water nanofluid-cooled micro channel with serpentine shaped fluid flow.