The study of protein nanoparticle interaction is very important for designing and fabricating nano composites which have potential medical applications. Bovine serum albumin (BSA), a major water soluble protein has wide range of physiological functions. In biomedical applications nanostructured metal oxides such as ZnO, TiO2 have drawn great attention for further studies as they are very safe to human beings and also for animals. Therefore, the interaction between BSA and ZnO nanoparticles (nps) and the effect of ZnO nps on the conformation of BSA has been analyzed by various spectroscopic techniques. The BSA-ZnO np conjugate made significant changes in BSA fluorescence emission parameters and thus confirmed the conformational changes.