Recently, biosynthesis of metal nanoparticles has drawn considerable attention due to environment friendly and sustainable methods. Herein, Bacillus subtilis TD 6 was selected as candidate for biosynthesis of silver nanoparticles (AgNPs). These nano sized silver particles (AgNPs) at concentration of 500 ppm were synthesized using maize and potato starch as both reducing and stabilizing agent. A solution containing AgNPs (500 ppm) was diluted with distilled water to 100 and 50 ppm and applied to cotton fabrics in presence of binder. The finished fabric characterization was carried out using UV Vis spectroscopy. The antibacterial activity of the treated fabrics loaded with AgNPs was evaluated against Escherichia coli (gram negative) and Staphylococcus aureus (gram positive) bacteria. Results explored that, binder retains excellent antibacterial properties even after 20 washing cycles reflecting the significance of binder in fixation of AgNPs deposits on the surface of the fabrics.