Carbon membranes have been studied in the last few years as a promising candidate for energy efficient gas separation processes in replacing the traditional membranes such as polymeric membranes. Carbon tubular membrane can be achieved by carbonizing polymeric tubular membrane under different process parameter. Carbonization is the most important step in the fabrication of carbon membrane and can be recognized as the heart of the carbon membrane production. The preparation and characterization of carbon membranes originated from Matrimid were studied. Matrimid based carbon tubular membrane will be fabricates and characterize in terms of its gas permeation properties. The carbonization temperature during the carbon membrane fabrication was studied. The polymer solution was coated on the surface of tubular ceramic tubes by using dip coating method. The polymer tubular membrane was then carbonized under Argon atmosphere at different carbonization temperature, 600, 750, and 850oC. Pure gas permeation tests were performed using CH4 and CO2 at room temperature with pressure 8 bars. Based on the results, the highest CO2/CH4 selectivity of 87.30 was obtained for carbon membrane prepared at 850oC.