Raw starch degrading amylase (RSDA) refers to amylase that can act directly on raw starch granules without gelatinization of starch. RSDA is commercially important enzyme useful in industry, since it saves much energy in gelatinizing starchy material. This study was aimed to isolating raw starch degrading microorganism and characterization of RSDA enzyme produced by selected microorganism. Two different samples, farm soil and cassava waste, were used as microorganism sources. The isolation was performed by