The noni juice was prepared by the use of honey. According to nutritional analysis, Calcium, Vitamin C and Carbohydrate were prominently present in the noni juice Moreover, the pH values of these juices products were acceptable and it was 3 Â± 1. As the alcohol percent of the noni juice products were very low and it was less than 1%, the final products of noni are called as fermented juice. No microbial population was observed on standard plate count media, EMB media, Mac-Conkey media, Baird Parker media and PDA media at overnight culture. . Antimicrobial activity showed that the juice can inhibit the growth of human pathogenic bacteria such as MRSA, Escherichia coli, Enterococcus faecalis and Bacillus cereus. The zone diameter of the product was investigated as 11mm for 50Î¼l concentration and 16mm for 100Î¼l conc: respectively for S.aureus. And for Escherichia coli, the inhibition zone diameters were observed as 13mm for 25Î¼l conc:, 15mm for 50Î¼l conc: and 18mm for 100Î¼l conc:. The sensitivity zone of the product was found as 12mm for 25Î¼l conc:, 15mm for 50Î¼l conc: and 22mm for 100Î¼l conc: respectively. The effect of the noni juice product on Bacillus cereus was seen as inhibition zone diameter 12mm, 15mm and 19mm for 25Î¼l, 50Î¼l and 100Î¼l respectively. Therefore, the noni juice product was more active against target pathogenic bacteria. According to anti-diabetic activity using mice model, the noni juice sample was highly significant like that of the positive control (Glibenclamide). Therefore, the product was potential to use as a routine drug as Glibenclamide. Due to the observational study on in vivo toxicity test, the noni juice product was safe to drink as nutraceutical food or as an alternative medicine.