In this study the distribution of binding sites of Concanavaline A (Con A), Wheat germ agglutinin (WGA), Ulex europaeus agglutinin (UEA) and Peanut agglutinin (PNA) lectins conjugated with fluorescence isothiocynate (FITC) with different specific carbohydrates were studied on cell surface of sperms of fertile and infertile men to determine whether the surface characters of the spermatozoa of fertile men differs from that of infertile men with or without obesity. A total of 255 infertile and 319 fertile men were involved in this study between Sep. 2006 and Dec. 2008. The body mass index (BMI) was measured in all fertile and infertile men. All semen parameters were assessed in both fertile and infertile men with or without obesity to identify the values that would distinguish fertile from infertile men. The results of this study showed that there is a clear significant difference between fertile and infertile men with or without obesity in regard to all semen parameters. On the other hand, all lectins used in this study were reacted differentially with the various components of the sperms of fertile and infertile men with or without obesity. This suggests that the obesity might alter the cell surface changes of sperms with increasing of BMI.