The present study describes antioxidant activities and cytotoxic activity of methanolic leaf extract of Blumea lanceolaria (Astereceae). The antioxidant activities were determined by in-vitro screening models; DPPH radical scavenging activity, Nitric oxide scavenging activity along with the estimation of total phenolic and flavfonoid content. From the result of DPPH radical scavenging activity and Nitric oxide scavenging activity the extract shows antioxidant activity with the IC50 values 55.56µg/ml and 70.60µg/ml respectively. The total phenolic and flavonoid content were found to be 60.20 gallic acid equivalent /gm of dried extract and 60 quercetin equivalent/gm of dried extract. The cytotoxic activity was investigated by MTT assay on HeLa (Human cervical cancer) cell line. The MTT assay suggest that the leaf extract of Blumea lanceolaria exhibited moderate cytotoxicity (20
100) against HeLa cell lines with IC50 95.63µg/ml.