The experiment was conducted at Department of Entomology, Khyber PakhtunKhwa Agricultural University Peshawar and Agricultural Research Institute, Mingora Swat Pakistan during summer, 2015 to find out the comparative efficacy of commonly used, commercially available acaricides i.e. Fenpyroximate, Spiromecifen, Pyridaben and Hexythiazox against Two Spotted Spider Mites (TSSM), Tetranychus urticae Koch in the lab and field conditions. Fenpyroximate, Spiromecifen and Pyridaben were proved the best among the tested ones for the control of TSSM based on their LC50 values and percent mortality in the lab and field experiment. All the tested acaricides caused significant mortality in the lab as well as in the field. Among the tested acaricides, Fenpyroximate, Spiromecifen and Pyridaben were found more effective against the two spotted spider mites than the other one. Mite population remained significantly lower in Fenpyroximate, Spiromecifen and Pyridaben treated plots (82-95 % mortality) then the Hexythiazox (17-30.83 % mortality, respectively). However, the Fenpyroximate showed the lowest LC50 value. The recommended application rates used in the field and the LT50 values further suggested judicious use effective acaricides.