Single crystals of L-Lysine doped (1 mol %) in organic, L-Prolinium picrate (LPP) and semi-organic, Potassium hydrogen phthalate (KHP) were successfully grown from solution by slow evaporation method at room temperature. The lattice parameters were confirmed by single crystal X-ray diffraction. The range of optical transmission are represented by recording UV-vis analysis. The Fourier Transform Infrared spectroscopy study confirms the incorporation of L-lysine into the LPP and KHP crystals. The thermal study indicates the dissociating nature of the crystal. The nonlinear optical property of the grown crystals have been confirmed by Kurtz-powder second harmonic generation test. The dopant of 1 mol % in organic material shows higher second harmonic generation than inorganic material.