Nanomaterials play a very important role in today’s material world. Nanoparticles are extensively studied for their optical, electronic, catalytic, magnetic, antimicrobial properties. In this present study, ZnO nanoparticles were synthesized using eco-friendly green method and conventional chemical method and the effect of green and chemical synthesized ZnO nanoparticle on structural and optical property were analyzed. The bio-solvent is extracted from citras sinuesis peel extract and analyzed by Fourier transform infrared spectroscopy (FTIR) and Gas chromatography Mass spectroscopy (GS-MS). Structural, morphological and optical properties of the synthesized nanoparticles were characterized by X–ray diffraction analysis (XRD), Scanning electron microscopy (SEM) and UV–Vis spectroscopy (UV-Vis) and Room temperature photoluminescence spectroscopy (RTPL). XRD result confirms the formation of hexagonal wurztile structure and enhanced crystallinty is observed for green synthesized ZnO nanoparticles. The synthesized nanoparticles produced by both methods have different size and morphology. Results revealed that the ZnO nanoparticles synthesized by green route shows enhanced structural and optical properties than chemically synthesized ZnO nanoparticles. Green synthesized nanomaterial finds extensive applications in photovoltaic and photocatalytic field.