Cellulolytic bacteria were isolated from manure wastes (cow dung) and degrading soil (municipal solid waste). Nine bacterial strains were screened the cellulolytic activities. Six strains showed clear zone formation on Berg’s medium. CMC (carboxyl methyl cellulose) and cellulose were used as substrates for cellulase activities. Among six strains, cd3 and mw7 were observed in quantitative measurement determined by dinitrosalicylic acid (DNS) method. Maximum enzyme producing activity showed 1.702mg/ml and 1.677mg/ml from cd3 and mw7 for 1% CMC substrate. On the other hand, it was expressed 0.563mg/ml and 0.415mg/ml for 1% cellulose substrate respectively. It was also studied for cellulase enzyme producing activity optimizing with kinetic growth parameters such as different carbon source including various concentration of cellulose, incubation time, temperature, and pH. Starch substrate showed 0.909mg/ml and 0.851mg/ml in enzyme producing activity. The optimum substrate concentration of cellulose was 0.25% for cd3 but 1% for mw7 showing the amount of reducing sugar formation 0.628mg/ml and 0.669mg/ml. The optimum incubation parameters for cd3 were 84 hours, 40C and pH 6. Mw7 also had optimum parameters 60 hours, 40 C and pH6.