The aim of the study is to establish new accurate Turbidometrical measurement of Sickle Hemoglobin using spectrophotometer instead of using naked eyes. Moreover, the study aimed to find out the most suitable filter and reducing reagent, which gives best result to improve the outcome of Solubility test. The study also intended to find out correlation between readings and previous transfusions as well as Jaundice. The study was carried out in Khartoum state among patients with sickle cell trait who were attending Khartoum educational hospital, Gafer Ibn Aouf Clinic and STAC International Centre Laboratory. Forty, 26 female and 14 male patients were recruited for the study. Of them, 34 were children and 6 were adults over 20 years old. There were also 30 normal persons recruited as control group for comparison. Results showed that 600 Nanometer is the best filter, which yielded highest light absorbance with significant statistical difference, and Na Meta-bisulphite is the best reducing agent because it produced turbidity more intense than Na Dithionate reagent. There is no significant correlation between reading and previous transfusion and jaundice. Therefore, the study recommend to use Na Meta-bisulphite for processing blood samples in Solubility test and to read the final reaction (Turbidity) by spectrophotometer using 600 Nanometer filter.