Disinfectants play an important role in health careassociated infection control by either minimizing or preventing microorganism dissemination. This article to study the morphological changes which may be related to the lose of antibiotic resistance after disinfectant exposure using SEM. Showed all isolates resistant to ampicillin, amoxicillin, cloxacillin, cephalexin, tetracycline, doxycycline, rifampin, chloramphenicol, trimethoprim cefotaxime and erythromycin, while one of burn isolates was susceptible for gentamicin, chloramphenicol and trimethoprim, and 15 of burn, 6 of wound, 5 of ear, and all urine isolates were susceptible to gentamicin using Kirby-Bauer method. The MICs of four common in use disinfectants (Hexatane, Dettol, Savlon and Povidone – Iodine) were determined for all isolates. The results showed that the MICs of Hexatane ranged from (64–512) μg/ml, Dettol (2048–16384) μg/ml, Savlon (4096:40960)–(32768:327680) μg/ml and for Povidone – Iodine MICs were (8192–32768) μg/ml. It has been found that burn and urine isolates were more resistant to disinfectants than wound and ear isolates. According to the effect of subMICs of disinfectants at different exposure patterns on antibiotic resistance, the results showed lose of resistance to tetracycline, doxycycline, rifampin, chloramphenicol, cefotaxime and trimethoprim in %72, %72, %68, %22, %28 and %36 of isolates, respectively. The results of SEM micrograph showed normal morphology and small sized bacteria with nub formation on some of them when exposed to dettol, and shape changes in cells with bulging in exposed to Povidone-iodine, while elongation and deformation were recorded in some cells in exposed to Savlon(chlorohexidine/ cetrimide) and Hexatane (chlorohexidine/ gluconate), respectively.