Nanotechnology is all about making products in nanometer scale to gain greatly enhanced material properties and functionality. Metal oxide fine particles are widely used in industrial applications as catalysts, pigments, fillers and so on. This paper reports the effect of addition of gelatin on the properties of TiO2 nanomaterials, synthesis and characterization. Rutile titanium dioxide nanoparticles were synthesized by a simple and cost-effective sol gel method using titanium isopropoxide as a precursor with 2- propanol and water as solvents. Gelatin was used as a gelling agent in the synthesis as it can provide long-term stability for nanoparticles by preventing particles agglomeration. XRD was used to study the crystalline phase and size of TiO2 nanomaterials. The X-ray diffraction studies indicate that the synthesized nanoparticles have only the rutile structure without the presence of any other phase impurities and the sizes of TiO2 nanoparticles synthesized without using and using gelatin are 40 nm and  20 nm respectively. Additionally, the TiO2 nanoparticles are characterized by FTIR spectroscopy and SEM. The results indicate that gelatin solution is a reliable and cheap green gelling agent that can be used as a matrix in the sol-gel method to synthesize TiO2 nanoparticles.