Brucellosis is an important re-emerging zoonotic disease. Apart from the public health hazard significant it has a great economic impact on both health and agricultural sectors. In health sector the burden of the disease is usually quantified in monetary and non –monitory terms. In agriculture the disease affects a range of domesticated animals resulting a huge economic loss in term of production and reproduction evolvement also the disease acts as trade impeder. This paper deals with the economic impacts of bovine brucellosis in Khartoum State, Sudan. Khartoum State as well as the country at large was proved to be endemic with both human and animal brucellosis. Many studies were carried out on the epidemiology of the disease, yet few studies were focused on the economic significant of the disease. In this work a comprehensive epidemiological survey covering the seven localities of the State was held to determine the situation of bovine brucellosis. A multi stage cluster sample was used to determine the prevalence rate in each locality where Rose Bengal Plate Test was used to identify the positive reactors. A weighed average of 25.1% prevalence rate was introduced to the economic model for assessment of the financial loss due to the disease. Accordingly the loss attributed to the disease was estimated at State, Locality and animal levels. Moreover, the cost due to reproduction, production and veterinary intervention was also quantified. The paper concluded that the disease constitutes a serious economic burden to the economy of the State and the producer in the absence of a formal control strategy. The paper recommended that there was an urgent need to formulate and implement livestock brucellosis control strategy in the State and the whole country for the benefit of both agriculture and health sectors.