Municipal Solid Waste (MSW), mainly Kitchen Waste (K) with Cow Dung (C) and Fungi Culture (F) can be used to generate energy which could save on the fossil fuels conventionally used as source of energy. In this study, the possibility was explored to mix Cow Dung with Fungi Culture for anaerobic digestion, so that energy can be generated as biogas and at the same time digested sludge can be used as fertilizer for agricultural applications. Pre-treatment of Kitchen Waste was done by alkali method. Anaerobic digestion (AD) was carried out in mesophilic temperature range of 30°C to 37°C with different fermentation slurries of 8 % total solids. Digestion was carried for a retention period of 60 days. The gas produced was collected by the downward displacement of water and was subsequently measured and analyzed. The overall results showed that blending of Kitchen waste with cow dung and fungi culture (Aspergillus flavus) had significant improvement on the biogas yield.